EXPRESSION OF SOLUBLE GUANYLYL CYCLASE - CATALYTIC ACTIVITY REQUIRES 2 ENZYME SUBUNITS

被引：145

作者：

HARTENECK, C ^{[1
]}

KOESLING, D ^{[1
]}

SOLING, A ^{[1
]}

SCHULTZ, G ^{[1
]}

BOHME, E ^{[1
]}

机构：

[1] FREE UNIV BERLIN, INST PHARMAKOL, THIELALLEE 67-73, W-1000 BERLIN 33, GERMANY

来源：

FEBS LETTERS | 1990年 / 272卷 / 1-2期

关键词：

COS cell; Expression; Soluble guanylyl cyclase;

D O I：

10.1016/0014-5793(90)80489-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Purified soluble guanylyl cyclase consists of two subunits (70 and 73 kDa) whose primary structures were recently determined. The availability of cDNA clones coding for either subunit allowed to study the question of the functional roles of the two subunits in expression experiments. Enzyme subunits were expressed in COS-7 cells by transfection with expression vectors containing the coding region for the 70 of 73 kDa subunit of the enzyme. No significant elevation in the activity of soluble guanylyl cyclase was observed in cells transfected with cDNA coding for one of the subunits. In contrast, transfection of cells with cDNAs coding for both subunits resulted in a marked increase in activity of soluble guanylyl cyclase. Enzyme activity was stimulated about 50-fold by sodium nitroprusside. The results indicate that formation of cyclic GMP by soluble guanylyl cyclase requires'both 70 and 73 kDa subunits. © 1990.

引用

页码：221 / 223

页数：3

共 15 条

[1]

ANDERSSON S, 1989, J BIOL CHEM, V264, P8222

[2] A MEMBRANE FORM OF GUANYLATE-CYCLASE IS AN ATRIAL NATRIURETIC PEPTIDE RECEPTOR [J].

CHINKERS, M ;

GARBERS, DL ;

CHANG, MS ;

LOWE, DG ;

CHIN, HM ;

GOEDDEL, DV ;

SCHULZ, S .

NATURE, 1989, 338 (6210) :78-83

[3]

CULLEN BR, 1987, METHOD ENZYMOL, V152, P684

[4] SOLUBLE GUANYLATE-CYCLASE PURIFIED FROM BOVINE LUNG CONTAINS HEME AND COPPER [J].

GERZER, R ;

BOHME, E ;

HOFMANN, F ;

SCHULTZ, G .

FEBS LETTERS, 1981, 132 (01) :71-74

[5] TISSUE DISTRIBUTION OF BETA-1-SUBUNIT AND BETA-2-SUBUNIT OF REGULATORY GUANINE NUCLEOTIDE-BINDING PROTEINS [J].

HINSCH, KD ;

TYCHOWIECKA, I ;

GAUSEPOHL, H ;

FRANK, R ;

ROSENTHAL, W ;

SCHULTZ, G .

BIOCHIMICA ET BIOPHYSICA ACTA, 1989, 1013 (01) :60-67

[6] PURIFICATION OF SOLUBLE GUANYLYL CYCLASE FROM BOVINE LUNG BY A NEW IMMUNOAFFINITY CHROMATOGRAPHIC METHOD [J].

HUMBERT, P ;

NIROOMAND, F ;

FISCHER, G ;

MAYER, B ;

KOESLING, D ;

HINSCH, KD ;

GAUSEPOHL, H ;

FRANK, R ;

SCHULTZ, G ;

BOHME, E .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1990, 190 (02) :273-278

[7]

KAMISAKI Y, 1986, J BIOL CHEM, V261, P7236

[8] THE PRIMARY STRUCTURE OF THE LARGER SUBUNIT OF SOLUBLE GUANYLYL CYCLASE FROM BOVINE LUNG - HOMOLOGY BETWEEN THE 2 SUBUNITS OF THE ENZYME [J].

KOESLING, D ;

HARTENECK, C ;

HUMBERT, P ;

BOSSERHOFF, A ;

FRANK, R ;

SCHULTZ, G ;

BOHME, E .

FEBS LETTERS, 1990, 266 (1-2) :128-132

[9] THE PRIMARY STRUCTURE OF THE 70 KDA SUBUNIT OF BOVINE SOLUBLE GUANYLATE-CYCLASE [J].

KOESLING, D ;

HERZ, J ;

GAUSEPOHL, H ;

NIROOMAND, F ;

HINSCH, KD ;

MULSCH, A ;

BOHME, E ;

SCHULTZ, G ;

FRANK, R .

FEBS LETTERS, 1988, 239 (01) :29-34

[10] ADENYLYL CYCLASE AMINO-ACID SEQUENCE - POSSIBLE CHANNEL-LIKE OR TRANSPORTER-LIKE STRUCTURE [J].

KRUPINSKI, J ;

COUSSEN, F ;

BAKALYAR, HA ;

TANG, WJ ;

FEINSTEIN, PG ;

ORTH, K ;

SLAUGHTER, C ;

REED, RR ;

GILMAN, AG .

SCIENCE, 1989, 244 (4912) :1558-1564

← 1 2 →