PRODUCTION OF ABIOTIC RECEPTORS BY MOLECULAR IMPRINTING OF PROTEINS

被引：63

作者：

BRACO, L ^{[1
]}

DABULIS, K ^{[1
]}

KLIBANOV, AM ^{[1
]}

机构：

[1] MIT,DEPT CHEM,CAMBRIDGE,MA 02139

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1990年 / 87卷 / 01期

关键词：

binding affinity; bioseparation; protein-ligand interaction; proteins in organic solvents; specificity;

D O I：

10.1073/pnas.87.1.274

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

When a protein is dissolved in a concentrated aqueous solution of a multifunctional organic compound, freeze-dried, and washed with an anhydrous organic solvent to remove the ligand, the resultant 'imprinted' protein preparation binds up to 30-fold more of the template compound in anhydrous solvents than the nonimprinted protein in the same solvent (and both proteins in water). These artificial receptors exhibit marked ligand selectivity as well as stability in anhydrous media. This phenomenon of molecular imprinting, demonstrated for several unrelated proteins and ligands, may be helpful in the development of unique bioadsorbents and, potentially, new biocatalysts.

引用

页码：274 / 277

页数：4

共 22 条

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