NON-ISOPEPTIDE-SELECTIVE ENDOTHELIN RECEPTORS IN HUMAN GIRARDI HEART-CELLS

被引：18

作者：

MIHARA, S

FUJIMOTO, M

机构：

[1] Shionogi Research Laboratories, Shionogi and Co., Ltd. 5-12-4, Fukushima-ku, Osaka, 553, Sagisu

来源：

LIFE SCIENCES | 1992年 / 50卷 / 03期

关键词：

D O I：

10.1016/0024-3205(92)90275-T

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

We characterized the endothelin (ET) receptor in Girardi heart (GH) cells derived from human atrium. The ET isopeptides ET-1, ET-2 and ET-3 induced the monotonous and long-lasting rise in cytosolic free Ca2+ concentration ([Ca2+]i) with almost the same potency in GH cells. Scatchard analysis of [I-125]ET-1 and [I-125]ET-3 binding revealed that GH cells have almost the same number of binding sites for either labeled ligand. All ET isopeptides displaced either [I-125]ET-1 or [I-125]ET-3 binding in GH cells almost equipotently. These results reveal that the functional ET receptors in GH cells are of the ET(B)-type. GH cells are the first cell line to be found to express the functional ET(B)-receptor.

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页码：219 / 226

页数：8

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