ANALYSIS OF AUTOANTIBODY BINDING TO 52-KD PARANEOPLASTIC CEREBELLAR DEGENERATION ASSOCIATED ANTIGEN EXPRESSED IN RECOMBINANT PROTEINS

被引：47

作者：

SAKAI, K ^{[1
]}

OGASAWARA, T ^{[1
]}

HIROSE, G ^{[1
]}

JAECKLE, KA ^{[1
]}

GREENLEE, JE ^{[1
]}

机构：

[1] VET ADM MED CTR,DEPT NEUROL,SALT LAKE CITY,UT 84148

来源：

ANNALS OF NEUROLOGY | 1993年 / 33卷 / 04期

关键词：

D O I：

10.1002/ana.410330407

中图分类号：

R74 [神经病学与精神病学];

学科分类号：

摘要：

A 52-kd neural antigen was reported to be recognized by anti-Purkinje cell antibodies in serum of a patient with paraneoplastic cerebellar degeneration associated with uterine carcinoma. In this study, we demonstrate that this neural antigen is recognized by antibodies known as anti-Purkinje cell antibody type I (PCAb Type I) and anti-YO. The latter's antigen is reported to be specific for the 62- to 64-kd antigen CDR62. Assuming that the 52-kd and 62- to 64-kd antigens share a common epitope(s) recognized by all of these antibodies, we examined the antigenic region on the 52-kd protein by immunoblots with deletion fragment proteins of the recombinant 52-kd protein. A major epitope was localized in the region of amino acid residues 94 to 133 of the 52-kd protein, which is the site of a leucine zipper motif. The potential pathogenicity of PCAb Type I is discussed.

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页码：373 / 380

页数：8

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