VISUALIZATION OF 2ND POLAR BODY CHROMOSOMES IN FERTILIZED AND ARTIFICIALLY ACTIVATED MOUSE OOCYTES TREATED WITH OKADAIC ACID

被引：14

作者：

DYBAN, AP ^{[1
]}

DESUTTER, P ^{[1
]}

DOZORTSEV, D ^{[1
]}

VERLINSKY, Y ^{[1
]}

机构：

[1] ILLINOIS MASONIC MED CTR,INST REPROD GENET,836 W WELLINGTON,CHICAGO,IL 60657

来源：

JOURNAL OF ASSISTED REPRODUCTION AND GENETICS | 1992年 / 9卷 / 06期

关键词：

2ND POLAR BODY; MOUSE; OKADAIC ACID; CYTOGENETICS; MEIOTIC NONDISJUNCTION;

D O I：

10.1007/BF01204256

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Objective: Our objective was to develop a new reliable method for cytogenetic analysis of the chromosome set in second polar bodies (PBs) from one-cell-stage mouse embryos. Setting: The study took place at the Reproductive Biology and Experimental Cytogenetics Laboratories. Methods: Oocytes from F1 hybrid and T6/T6 mice were fertilized in vitro and artificially activated with ethanol. Zygotes, parthenogenetic embryos, and isolated second PBs were treated with 10 muM okadaic acid (OA) for 1-2 hr, further cultured in plain medium, and fixed. Chromosomal preparations were made and C-banded, and the number of chromosomes in second PBs and embryos was counted. Results: OA-induced nuclear envelope breakdown in pronuclei as well as in second PB nuclei. Countable chromosome plates were obtained in 92-93% of second PBs treated 4-4.5 hr after activation. The T6 marker chromosome could easily be recognized in second PBs from T6/T6 mice. A haploid set of chromosomes was obtained in 18 of 19 isolated second PBs treated with OA 4-5 hr after activation. Conclusion: Treatment of second PBs with OA allows visualization of the PB chromosomes. Cytogenetic analysis of the second PB and the corresponding oocyte constitutes a new approach for the study of meiotic nondisjunction in experimental cytogenetics. The chromosomal study of isolated second PBs seems to be promising for clinical preimplantation cytogenetics.

引用

页码：572 / 579

页数：8

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