VISUALIZATION OF 2ND POLAR BODY CHROMOSOMES IN FERTILIZED AND ARTIFICIALLY ACTIVATED MOUSE OOCYTES TREATED WITH OKADAIC ACID

被引：13

作者：

DYBAN, AP ^{[1
]}

DESUTTER, P ^{[1
]}

DOZORTSEV, D ^{[1
]}

VERLINSKY, Y ^{[1
]}

机构：

[1] ILLINOIS MASONIC MED CTR,INST REPROD GENET,836 W WELLINGTON,CHICAGO,IL 60657

来源：

JOURNAL OF ASSISTED REPRODUCTION AND GENETICS | 1992年 / 9卷 / 06期

关键词：

2ND POLAR BODY; MOUSE; OKADAIC ACID; CYTOGENETICS; MEIOTIC NONDISJUNCTION;

D O I：

10.1007/BF01204256

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Objective: Our objective was to develop a new reliable method for cytogenetic analysis of the chromosome set in second polar bodies (PBs) from one-cell-stage mouse embryos. Setting: The study took place at the Reproductive Biology and Experimental Cytogenetics Laboratories. Methods: Oocytes from F1 hybrid and T6/T6 mice were fertilized in vitro and artificially activated with ethanol. Zygotes, parthenogenetic embryos, and isolated second PBs were treated with 10 muM okadaic acid (OA) for 1-2 hr, further cultured in plain medium, and fixed. Chromosomal preparations were made and C-banded, and the number of chromosomes in second PBs and embryos was counted. Results: OA-induced nuclear envelope breakdown in pronuclei as well as in second PB nuclei. Countable chromosome plates were obtained in 92-93% of second PBs treated 4-4.5 hr after activation. The T6 marker chromosome could easily be recognized in second PBs from T6/T6 mice. A haploid set of chromosomes was obtained in 18 of 19 isolated second PBs treated with OA 4-5 hr after activation. Conclusion: Treatment of second PBs with OA allows visualization of the PB chromosomes. Cytogenetic analysis of the second PB and the corresponding oocyte constitutes a new approach for the study of meiotic nondisjunction in experimental cytogenetics. The chromosomal study of isolated second PBs seems to be promising for clinical preimplantation cytogenetics.

引用

页码：572 / 579

页数：8

共 24 条

[1]

Kaufman M.H., Early Mammalian Development: Parthenogenetic Studies, (1983)

[2]

Dyban A.P., Baranov V.S., Cytogenetics of Mammalian Embryonic Development, (1987)

[3]

Pellestor F., Frequency and distribution of aneuploidy in human female gametes, Hum Genet, 86, pp. 283-288, (1991)

[4]

Plachot M., Chromosome analysis of oocytes and embryos, Preimplantation Genetics, pp. 103-112, (1991)

[5]

Modlinski J., McLaren A., A method for visualizing the chromosomes of the second polar body of the mouse egg, J Embryol Exp Morphol, 60, pp. 93-97, (1980)

[6]

Verlinsky Y., Cieslak J., Wolf G., Rechitsky S., Strom C., Lifchez A., Ginsberg N., Applebaum M., Dyban A., Polar body sampling, Prenatal Diag, (1992)

[7]

Cohen P., Holmes C.F.B., Tsutikani Y., Okadaic acid: A new probe for the study of cellular regulation, Trends Biochem Sci, 15, pp. 98-102, (1990)

[8]

Schonthal A., Okadaic acid—a valuable new tool for the study of signal transduction and cell cycle regulation?, New Biol, 4, pp. 16-21, (1992)

[9]

Steinmann K.E., Belinsky G.S., Lee D., Schlegel R., Chemically induced premature mitosis: Differential response in rodent and human cells and the relationship to cyclin B synthesis and p34 (cdc2)/cyclin B complex formation, Proc Natl Acad Sci USA, 88, pp. 6843-6847, (1991)

[10]

Vandre D.D., Wills V.L., Inhibition of mitosis by okadaic acid: Possible involvement of a protein phosphatase 2A in the transition from metaphase to anaphase, J Cell Sci, 101, pp. 79-91, (1992)

← 1 2 3 →