MASS-SPECTROMETRIC ANALYSIS OF A GPI-ANCHORED PROTEIN - THE SCRAPIE PRION PROTEIN

被引：10

作者：

BALDWIN, MA

BURLINGAME, AL

PRUSINER, SB

机构：

[1] UNIV CALIF SAN FRANCISCO, SCH PHARM, DEPT PHARMACEUT CHEM, SAN FRANCISCO, CA 94143 USA

[2] UNIV CALIF SAN FRANCISCO, SCH MED, DEPT NEUROL, SAN FRANCISCO, CA 94143 USA

[3] UNIV CALIF SAN FRANCISCO, SCH MED, DEPT BIOCHEM & BIOPHYS, SAN FRANCISCO, CA 94143 USA

来源：

TRAC-TRENDS IN ANALYTICAL CHEMISTRY | 1993年 / 12卷 / 06期

基金：

美国国家科学基金会;

关键词：

D O I：

10.1016/0165-9936(93)87063-4

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Diverse mass spectrometric methods have been used for the analysis of the scrapie prion protein, a membrane bound protein anchored via a glycosylphosphatidylinositol (GPI) group and available in quantities of only a few nanomoles. The sensitivity, specificity and flexibility of modern mass spectrometric techniques were essential in confirming the amino acid sequence and in characterizing the nature of the post-translational modifications including the GPI anchor. New methods are described for the determination of the structure of the GPI.

引用

收藏

页码：239 / 248

页数：10

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