STUDIES OF THE INTERACTION OF A META-HYDROXY ANALOG OF HOECHST-33258 WITH DNA BY MELTING TEMPERATURE, FOOTPRINTING AND HIGH-RESOLUTION H-1-NMR SPECTROSCOPY

被引：17

作者：

EBRAHIMI, SES

PARKINSON, JA

FOX, KR

MCKIE, JH

BARBER, J

DOUGLAS, KT

机构：

[1] UNIV MANCHESTER,DEPT PHARM,MANCHESTER M13 9PL,LANCS,ENGLAND

[2] UNIV EDINBURGH,DEPT CHEM,EDINBURGH EH9 3JJ,MIDLOTHIAN,SCOTLAND

[3] UNIV SOUTHAMPTON,SCH BIOL SCI,DEPT PHYSIOL & PHARMACOL,SOUTHAMPTON SO9 3TU,HANTS,ENGLAND

来源：

JOURNAL OF THE CHEMICAL SOCIETY-CHEMICAL COMMUNICATIONS | 1992年 / 19期

关键词：

D O I：

10.1039/c39920001398

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A new analogue of Hoechst 33258 1 with a small structural change, i.e. the phenolic group being located meta rather than para, has been shown by footprinting, DNA melting studies and high-field H-1 NMR spectroscopy to bind to the AATT sequences of DNA in a manner grossly similar to Hoechst 33258, although analysis by H-1 NMR spectroscopy indicates that the phenolic end of the ligand could be prised away from the minor groove relative to Hoechst 33258, and that the major groove structure of d(CGCGAATTCGCG)2 is significantly perturbed by the binding of this Hoechst 33258 analogue.

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页码：1398 / 1399

页数：2

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