Nitrogen management is important in determining cotton (Gossypium hirsutum L.) yield and fiber quality. Plant tissue tests are often used to estimate cotton N status and determine if further fertilization is needed during the growing season. This study was conducted to examine the relationship between plant tissue tests of petiole NO3 concentration and leaf N content and cotton performance as measured by vegetative growth (i.e., leaf area) and bell load. Nitrogen rates of 0, 18, 36, 72, and 144 mM N pot(-1) were applied at 2-wk intervals beginning 13 and 26 d after emergence in 1987 and 1988 through bell maturity to a greenhouse-grown cultivar, Stoneville 213. The strong relationship between leaf N and leaf area and boll number (r(2) = 0.80 and r(2) = 0.89) 1 wk after first flower appearance suggests that the transition period between vegetative and boll development is a critical time to assess cotton's N status in relation to yield. Nitrogen deficits had little effect on bell weight, boll period duration, and number of main stem nodes. Although boll weight decreased with increasing N deficit, final boll weight of the high-N plants were within 20% of the plants receiving low N supply. The relationship between leaf N and petiole NO3 content, was best described by Y = a + bX(C); however, with this function only one-half of the variation in leaf N was accounted for by variation in petiole NO3 content (r(2) = 0.48). Leaf area and boll number were poorly correlated with petiole NO3 content. Leaf N content was a better predictor of cotton vegetative growth (as indicated by LAI) and bell number than petiole NO3 content, particularly when measurements are made 1 wk after first flower