CLONING AND CHARACTERIZATION OF THE CALMODULIN-ENCODING GENE FROM PNEUMOCYSTIS-CARINII

被引：14

作者：

FLETCHER, LD ^{[1
]}

BERGER, LC ^{[1
]}

TIDWELL, RR ^{[1
]}

DYKSTRA, CC ^{[1
]}

机构：

[1] UNIV N CAROLINA,SCH MED,DEPT PATHOL,CB 7525,811 BRINKHOUS,CHAPEL HILL,NC 27599

来源：

GENE | 1993年 / 129卷 / 02期

关键词：

NUCLEOTIDE SEQUENCE; OPPORTUNISTIC INFECTIONS; AIDS; AMINO ACID HOMOLOGY; FUNGI;

D O I：

10.1016/0378-1119(93)90285-B

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Complete cDNA and genomic clones for the CaM gene encoding calmodulin (CaM) from Pneumocystis carinii have been isolated from rat and characterized. The nucleotide (nt) sequence contains an open reading frame interrupted by three introns, that encodes a protein of 152 amino acids. The predicted CaM protein of P. carinii shares a high degree of homology with other known CaM proteins.

引用

页码：307 / 308

页数：2

共 7 条

[1] STRUCTURE OF CALMODULIN REFINED AT 2.2 A RESOLUTION [J].

BABU, YS ;

BUGG, CE ;

COOK, WJ .

JOURNAL OF MOLECULAR BIOLOGY, 1988, 204 (01) :191-204

[2] ISOLATION OF THE YEAST CALMODULIN GENE - CALMODULIN IS AN ESSENTIAL PROTEIN [J].

DAVIS, TN ;

URDEA, MS ;

MASIARZ, FR ;

THORNER, J .

CELL, 1986, 47 (03) :423-431

[3] ISOLATION AND IDENTIFICATION OF 6 PNEUMOCYSTIS-CARINII GENES UTILIZING CODON BIAS [J].

FLETCHER, LD ;

BERGER, LC ;

PEEL, SA ;

BARIC, RS ;

TIDWELL, RR ;

DYKSTRA, CC .

GENE, 1993, 129 (02) :167-174

[4] STRUCTURAL AND FUNCTIONAL-PROPERTIES OF CALMODULIN FROM THE EUKARYOTIC MICROORGANISM DICTYOSTELIUM-DISCOIDEUM [J].

MARSHAK, DR ;

CLARKE, M ;

ROBERTS, DM ;

WATTERSON, DM .

BIOCHEMISTRY, 1984, 23 (13) :2891-2899

[5]

MEANS AR, 1988, CALMODULIN MOL ASPEC, P17

[6]

RASMUSSEN CD, 1990, J BIOL CHEM, V265, P13767

[7] ANALYSIS AND INVIVO DISRUPTION OF THE GENE CODING FOR CALMODULIN IN SCHIZOSACCHAROMYCES-POMBE [J].

TAKEDA, T ;

YAMAMOTO, M .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (11) :3580-3584

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