ISOLATION AND CHARACTERIZATION OF CLONED FRAGMENTS OF BACTERIOPHAGE-P1 DNA

被引：59

作者：

MURAL, RJ

CHESNEY, RH

VAPNEK, D

KROPF, MM

SCOTT, JR

机构：

[1] EMORY UNIV HOSP,DEPT MICROBIOL,ATLANTA,GA 30322

[2] UNIV GEORGIA,DEPT MICROBIOL,PROGRAM GENET,ATHENS,GA 30602

[3] UNIV GEORGIA,DEPT BIOCHEM,ATHENS,GA 30602

[4] UNIV GEORGIA,DEPT ZOOL,ATHENS,GA 30602

来源：

VIROLOGY | 1979年 / 93卷 / 02期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1016/0042-6822(79)90243-5

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Fragments of PI DNA generated by endo·R·EcoRI and endo·R·BamHI were mixed with appropriate cloning vectors (ColElApr, pBR313, pBR322) ligated in vitro and used to transform a nonsuppressing strain of Escherichia coli. In this way clones of 9 of the 26 EcoRI fragments and 5 of the 14 BamHI fragments were obtained. Marker rescue tests were used to ascertain which regions of the P1 genome were contained on the various DNA fragments. These cloned fragments are useful as probes for specific regions of the P1 genome. © 1979.

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页码：387 / 397

页数：11

相关论文

共 43 条

[1] MOLECULAR CLONING OF RESTRICTION FRAGMENTS AND CONSTRUCTION OF A PHYSICAL AND GENETIC MAP OF ESCHERICHIA-COLI PLASMID-R538-1 [J].

ALTON, NK ;

VAPNEK, D .

PLASMID, 1978, 1 (03) :388-404

[2] HOST SPECIFICITY OF DNA PRODUCED BY ESCHERICHIA-COLI .1. HOST CONTROLLED MODIFICATION OF BACTERIOPHAGE LAMBDA [J].

ARBER, W ;

DUSSOIX, D .

JOURNAL OF MOLECULAR BIOLOGY, 1962, 5 (01) :18-+

[3] PHYSICAL MAPPING OF BGLII, BAMHI, ECORI, HINDIII AND PSTI RESTRICTION FRAGMENTS OF BACTERIOPHAGE-P1 DNA [J].

BACHI, B ;

ARBER, W .

MOLECULAR & GENERAL GENETICS, 1977, 153 (03) :311-324

[4] PEDIGREES OF SOME MUTANT STRAINS OF ESCHERICHIA-COLI K-12 [J].

BACHMANN, BJ .

BACTERIOLOGICAL REVIEWS, 1972, 36 (04) :525-557

[5] CONSTRUCTION AND CHARACTERIZATION OF NEW CLONING VEHICLES .1. AMPICILLIN-RESISTANT DERIVATIVES OF PLASMID PMB9 [J].

BOLIVAR, F ;

RODRIGUEZ, RL ;

BETLACH, MC ;

BOYER, HW .

GENE, 1977, 2 (02) :75-93

[6] CONSTRUCTION AND CHARACTERIZATION OF NEW CLONING VEHICLES .2. MULTIPURPOSE CLONING SYSTEM [J].

BOLIVAR, F ;

RODRIGUEZ, RL ;

GREENE, PJ ;

BETLACH, MC ;

HEYNEKER, HL ;

BOYER, HW ;

CROSA, JH ;

FALKOW, S .

GENE, 1977, 2 (02) :95-113

[7]

BOVRE K, 1971, METHODS ENZYMOLOGY D, V21

[8]

CATTERALL JF, 1977, J BACTERIOL, V129, P1118

[9] SUPERINFECTION IMMUNITY AND PROPHAGE REPRESSION IN PHAGE-P1 .2. MAPPING OF IMMUNITY-DIFFERENCE AND AMPICILLIN-RESISTANCE LOCI OF P1 AND PHIAMP [J].

CHESNEY, RH ;

SCOTT, JR .

VIROLOGY, 1975, 67 (02) :375-384

[10] NONCHROMOSOMAL ANTIBIOTIC RESISTANCE IN BACTERIA - GENETIC TRANSFORMATION OF ESCHERICHIA-COLI BY R-FACTOR DNA [J].

COHEN, SN ;

CHANG, ACY ;

HSU, L .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1972, 69 (08) :2110-&

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