SIMPLE METHOD FOR PURIFICATION OF PHOSPHOLIPASE A FROM COBRA VENOM

被引：28

作者：

BRAGANCA, BM

SAMBRAY, YM

GHADIALLY, RC

机构：

[1] Biochemistry Division, Cancer Research Institute, Tata Memorial Centre, Bombay

来源：

TOXICON | 1969年 / 7卷 / 02期

关键词：

D O I：

10.1016/0041-0101(69)90078-6

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

A simple method is described for the purification of phospholipase A. The enzyme preparation obtained was purified 20-fold, and was homogeneous on starch gel electrophoresis. The yield was 5 to 6 per cent with a recovery of 60 to 70 per cent of the total units present in the starting material. © 1969.

引用

收藏

页码：151 / +

页数：1

相关论文

共 15 条

[1] CHROMATOGRAPHY OF RATTLESNAKE VENOM - A SEPARATION OF 3 PHOSPHODIESTERASES [J].

BOMAN, HG ;

KALETTA, U .

BIOCHIMICA ET BIOPHYSICA ACTA, 1957, 24 (03) :619-631

[2]

BRAGANCA BM, 1967, BIOCHIM BIOPHYS ACTA, V136, P508

[3] MULTIPLE FORMS OF COBRA VENOM PHOSPHOLIPASE A [J].

BRAGANCA, BM ;

SAMBRAY, YM .

NATURE, 1967, 216 (5121) :1210-&

[4] GLYCOPROTEINS AS COMPONENTS OF LETHAL FACTORS IN COBRA VENOM (NAJA NAJA) [J].

BRAGANCA, BM ;

PATEL, NT .

CANADIAN JOURNAL OF BIOCHEMISTRY, 1965, 43 (07) :915-&

[5] ACTION OF SNAKE VENOM ON ACETYLCHOLINE SYNTHESIS IN BRAIN [J].

BRAGANCA, BM ;

QUASTEL, JH .

NATURE, 1952, 169 (4304) :695-697

[6] HEMOLYSIS + SPLITTING OF HUMAN ERYTHROCYTE PHOSPHOLIPIDS BY SNAKE VENOMS [J].

CONDREA, E ;

DEVRIES, A .

BIOCHIMICA ET BIOPHYSICA ACTA, 1964, 84 (01) :60-&

[7]

DE S. S., 1944, Annals of Biochemistry and Experimental Medicine, V4, P45

[8]

DELEZENNE C, 1911, CR HEBD ACAD SCI, V152, P790

[9] PHOSPHOLIPASE B IN SNAKE VENOMS + BEE VENOM [J].

DOERY, HM ;

PEARSON, JE .

BIOCHEMICAL JOURNAL, 1964, 92 (03) :599-&

[10] INACTIVATION OF CROTOXIN BY GROUP-SPECIFIC REAGENTS [J].

FRAENKELCONRAT, H ;

FRAENKELCONRAT, J .

BIOCHIMICA ET BIOPHYSICA ACTA, 1950, 5 (01) :98-104