ANALYSIS OF OLIGONUCLEOTIDES BY CAPILLARY GEL-ELECTROPHORESIS

被引：90

作者：

PAULUS, A ^{[1
]}

OHMS, JI ^{[1
]}

机构：

[1] BECKMAN INSTRUMENTS INC,DEPT RES,DIV SPINCO,PALO ALTO,CA 94304

来源：

JOURNAL OF CHROMATOGRAPHY | 1990年 / 507卷

关键词：

D O I：

10.1016/S0021-9673(01)84187-X

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Analysis of oligonucleotides with gel-filled capillary columns is a fast, efficient and automated way to check their purity. Gel-filled columns were optimized to separate oligonucleotides which were 20-50 nucleotides in length. The influence of sample size on resolution is discussed. A comparison between capillary gel electrophoresis and slab gel electrophoresis in terms of analysis time per sample is made. In addition to homopolymeric samples, the separation of heteropolymeric oligonucleotides with one nucleotide difference in size is presented and suggests the possibility of using capillary gel electrophoresis as a tool for DNA sequencing. © 1990.

引用

页码：113 / 123

页数：11

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