LB FILM IMMOBILIZED IMMUNOSENSOR WITH A CHARGE DETECTION TYPE AMPLIFIER

被引：4

作者：

KATSUBE, T ^{[1
]}

YAJI, T ^{[1
]}

SUZUKI, K ^{[1
]}

KOBAYASHI, S ^{[1
]}

KAWAGUCHI, T ^{[1
]}

SHIRO, T ^{[1
]}

机构：

[1] TEIJIN LTD,TOKYO RES CTR,HINO,TOKYO 191,JAPAN

来源：

APPLIED SURFACE SCIENCE | 1988年 / 33-4卷

关键词：

Antigens; -; Enzymes--Immobilization; Immunology--Sensors; Proteins;

D O I：

10.1016/0169-4332(88)90452-7

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

In order to fabricate a miniaturized solid-state sensor, a novel potentiometric immunosensor was designed by incorporating a new amplification system of charge detection type and an electrode covered with immunoglobin-G (IgG, antigen) immobilized in a Langmuir-Blodgett (LB) film. A surface potential change of 1 μV generated by the immuno-reaction between IgG and anti-IgG (antibody) could be measured with the amplification system, which made it possible to detect the anti-IgG with concentrations less than 5×10-8M. The intensity of surface potential change observed in the reaction was successfully correlated with the activity of the immobilized IgG by using luminescent enzyme immunoassay.

引用

页码：1332 / 1338

页数：7