A SIMPLE DIAMYLASE PROCEDURE FOR THE ESTIMATION OF ALPHA-AMYLASE AND DIASTATIC ACTIVITY

被引:30
作者
ETOKAKPAN, OU
PALMER, GH
机构
[1] HERIOT WATT UNIV,INT CTR BREWING & DISTILLING,EDINBURGH EH1 1HX,MIDLOTHIAN,SCOTLAND
[2] DEPT BIOL SCI,EDINBURGH EH1 1HX,SCOTLAND
关键词
barley; dextrinizing units; diastatic power; enzyme inhibition; mercuric chloride; sorghum; β‐amylase;
D O I
10.1002/j.2050-0416.1990.tb01020.x
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
Mercuric chloride was used to selectively inhibit the sulphydryl function of β‐amylase, enabling the dextrinizing activities of α‐amylase and the Diastatic Power (total reducing power activity) to be calculated from a single extract of malt by one assay method. Using commercial malts, there was a 98% and 96% correlation between results obtained by this method and the recommended methods of the Institute of Brewing (IOB) for the determination of Diastatic Power (DP) and Dextrinizing Unit (DU). 1990 The Institute of Brewing & Distilling
引用
收藏
页码:89 / 91
页数:3
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