YEAST RIBOSOMAL-PROTEINS .2. ISOLATION AND CHARACTERIZATION OF 14-RIBOSOMAL PROTEINS FROM SMALL SUBUNITS OF YEAST

被引：14

作者：

HIGO, K ^{[1
]}

OTAKA, E ^{[1
]}

机构：

[1] HIROSHIMA UNIV, NUCL MED & BIOL RES INST, DEPT BIOCHEM & BIOPHYS, HIROSHIMA 734, JAPAN

来源：

BIOCHEMISTRY | 1979年 / 18卷 / 19期

关键词：

D O I：

10.1021/bi00586a024

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A method for preparation of a large amount of ribosomal subunits from Saccharomyces cerevisiae by a Ti-15 zonal rotor is described. The proteins of the small subunits (ca. 50000 A260 units) were separated into 22 fractions by chromatography on carboxymethylcellulose columns. Fourteen proteins were then purified from the ten chromatographic fractions by filtration through Sephadex G-100 or Sephacryl S-200. The isolated proteins are YP 6, YP 7, YP 9, YP 12, YP 14′, YP 14”, YP 28, YP 38, YP 45, YP 50, YP 52, YP 58, YP 63, and YP 70. The molecular weights and amino acid compositions of these proteins are presented. © 1979, American Chemical Society. All rights reserved.

引用

页码：4191 / 4196

页数：6

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