COMPARISON OF NUCLEIC-ACID HYBRIDIZATION AND NUCLEIC-ACID AMPLIFICATION USING CONSERVED SEQUENCES FROM THE 5' NONCODING REGION FOR DETECTION OF BOVINE VIRAL DIARRHEA VIRUS

被引：38

作者：

RIDPATH, JF ^{[1
]}

BOLIN, SR ^{[1
]}

KATZ, J ^{[1
]}

机构：

[1] USDA,ANIM & PLANT HLTH INSPECT SERV,NATL VET SERV LABS,AMES,IA 50010

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 1993年 / 31卷 / 04期

关键词：

D O I：

10.1128/JCM.31.4.986-989.1993

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Primers and probes derived from conserved sequences located in the 5' noncoding region of pestiviruses were evaluated for detection of bovine viral diarrhea virus. With these reagents, hybridization and polymerase chain reaction tests detected 62 of 90 and 90 of 90 bovine viral diarrhea virus isolates, respectively. A quick lysis method for preparing RNA for use in polymerase chain reaction amplification also was evaluated.

引用

页码：986 / 989

页数：4

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