ISOLATION AND SELECTION OF AN L-AMINOACYLASE-PRODUCING BACTERIUM, PSEUDOMONAS SP BA2

被引：7

作者：

SANTOYO, AB ^{[1
]}

RODRIGUEZ, JB ^{[1
]}

INIESTA, FM ^{[1
]}

GOMEZ, EG ^{[1
]}

TERUEL, MLA ^{[1
]}

ROJO, IA ^{[1
]}

机构：

[1] FAC VET MURCIA,MURCIA,SPAIN

来源：

LETTERS IN APPLIED MICROBIOLOGY | 1994年 / 19卷 / 06期

关键词：

D O I：

10.1111/j.1472-765X.1994.tb00982.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The enrichment culture method was used to detect and isolate L-aminoacylase-producing bacteria from soil, using N-acetyl-L-alanine as inducer and substrate. Isolated bacterial strains were screened for growth and enzyme activity. Strain BA2 displayed both the highest intracellular L-aminoacylase activity and the most profuse growth. Furthermore, BA2 cells did not show any D-aminoacylase activity. This strain was an obligately aerobic rod-shaped bacterium and stained Gram-negative, and was therefore identified as Pseudomonas. Its morphological and biochemical characteristics corresponded to those of Pseudomonas fluorescens biovar I.

引用

页码：461 / 465

页数：5

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