CARBOHYDRATE SPECIFICITY OF THE ESCHERICHIA-COLI P-PILUS PAPG PROTEIN IS MEDIATED BY ITS N-TERMINAL PART

被引：6

作者：

HANSSON, L

WALLBRANDT, P

ANDERSSON, JO

BYSTROM, M

BACKMAN, A

CARLSTEIN, A

ENQUIST, K

LONN, H

OTTER, C

STROMQVIST, M

机构：

[1] SYMBICOM AB,S-90736 UMEA,SWEDEN

[2] SYMBICOM AB,S-22370 LUND,SWEDEN

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS | 1995年 / 1244卷 / 2-3期

关键词：

BACTERIAL ADHESION; FUSION PROTEIN; CARBOHYDRATE BINDING;

D O I：

10.1016/0304-4165(95)00028-A

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The adherence of pyelonephritic Escherichia coli isolates to mammalian host cells is mediated by the P-pili structures on the bacterial surface. The protein constituting the distal part of the pill structure, papG, interacts with glycan receptors on the host cell. Variation in specificity for different glycoconjugates between the isolates, that may reflect variation in host tropism, has been correlated to three different classes of papG. Truncated variants of the class I, II and III papG adhesins were produced as fusion protein in E. coli and analysed for carbohydrate binding. The results showed that both carbohydrate binding and specificity of the papG adhesin resided in a linear part of the N-terminus of the protein.

引用

页码：377 / 383

页数：7

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