EVALUATION OF COMMERCIAL FLUORESCEIN ISOTHIOCYANATES USED IN FLUORESCENT ANTIBODY STUDIES

被引：19

作者：

CHERRY, WB

MCKINNEY, RM

EMMEL, VM

SPILLANE, JT

HEBERT, GA

PITTMAN, B

机构：

[1] National Communicable Disease Center, Public Health Service, U. S. Department of Health, Education, and Welfare, Atlanta, GA

[2] Technical Development Laboratories, National Communicable Disease Center, Savannah, GA

[3] Department of Anatomy, University of Rochester Medical Center, Rochester, NY

[4] National Communicable Disease Center, Savannah, GA

[5] National Communicable Disease Center, Atlanta, GA

来源：

STAIN TECHNOLOGY | 1969年 / 44卷 / 04期

关键词：

D O I：

10.3109/10520296909063347

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Commercial preparations of fluorescein isothiocyanate (FITC) for immunofluorescence applications were obtained from 12 sources and examined for purity by quantitative infrared spectrophotometry and by labeling efficiency for bovine serum albumin (BSA). Quantitative photometric measurements were made of nonspecific staining (NSS) produced by conjugates prepared from the dyes. The purity of FITC from different sources was highly variable. The risk of NSS appears to increase as the purity of the dye decreases. In immunofluorescence applications it is desirable to use the purest FITC available in order to obtain conjugates with minimum NSS. It is recommended that 70% FITC, as determined by BSA labeling efficiency, be accepted as the minimum purity for immunofluorescence applications. © 1969 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.

引用

页码：179 / &

共 5 条

[1]

HEBERT GA, 1967, J IMMUNOL, V98, P1204

[2]

MCKINNEY RM, 1964, J IMMUNOL, V93, P232

[3] A SIMPLE METHOD FOR DETERMINING LABELING EFFICIENCY OF FLUORESCEIN ISOTHIOCYANATE PRODUCTS [J].

MCKINNEY, RM ;

SPILLANE, JT ;

PEARCE, GW .

ANALYTICAL BIOCHEMISTRY, 1966, 14 (03) :421-&

[4] DETERMINATION OF PURITY OF FLUORESCEIN ISOTHIOCYANATES [J].

MCKINNEY, RM ;

PEARCE, GW ;

SPILLANE, JT .

ANALYTICAL BIOCHEMISTRY, 1964, 7 (01) :74-&

[5]

PITTMAN B, 1967, J IMMUNOL, V98, P1196

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