CONSTRUCTION OF A UMUDC OPERON SUBSTITUTION MUTATION IN ESCHERICHIA-COLI

被引：107

作者：

WOODGATE, R

机构：

[1] Section on Viruses and Cellular Biology, National Institute of Child Health and Human Development, Bethesda, MD 20892, Building 6

来源：

MUTATION RESEARCH | 1992年 / 281卷 / 03期

关键词：

ESCHERICHIA-COLI; SOS MUTAGENESIS; UMUDC OPERON; LAMBDA-GTL-LAMBDA-B; GENE REPLACEMENT;

D O I：

10.1016/0165-7992(92)90012-7

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Using a specialized transducing lambda phage, the umuDC operon of Escherichia coli was deleted and replaced with the chloramphenicol acetyltransferase gene. The DELTA(umuDC)595::cat mutation was subsequently transferred by generalized P1 transduction into a variety of genetic backgrounds. It is concluded that the UmuD,C proteins, which are normally required for inducible mutagenesis, are not essential for cell survival.

引用

页码：221 / 225

页数：5

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