A POTASSIUM CHANNEL TOXIN FROM THE SECRETION OF THE SEA-ANEMONE BUNODOSOMA-GRANULIFERA - ISOLATION, AMINO-ACID-SEQUENCE AND BIOLOGICAL-ACTIVITY

被引：84

作者：

ANEIROS, A

GARCIA, I

MARTINEZ, JR

HARVEY, AL

ANDERSON, AJ

MARSHALL, DL

ENGSTROM, A

HELLMAN, U

KARLSSON, E

机构：

[1] BIOMED CTR,DEPT BIOCHEM,BOX 576,S-75123 UPPSALA,SWEDEN

[2] INST INVEST FUNDAMENTALES CEREBRO,HAVANA,CUBA

[3] UNIV STRATHCLYDE,INST DRUG RES,DEPT PHYSIOL & PHARMACOL,GLASGOW G1 1XW,SCOTLAND

[4] LUDWIG INST CANC RES,UPPSALA,SWEDEN

[5] BIOMED CTR,DEPT IMMUNOL,UPPSALA,SWEDEN

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1993年 / 1157卷 / 01期

关键词：

POTASSIUM CHANNEL TOXIN; AMINO ACID SEQUENCE; BIOLOGICAL ACTIVITY; (SEA ANEMONE);

D O I：

10.1016/0304-4165(93)90082-J

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A peptide toxin affecting potassium channels was isolated from the sea anemone Bunodosoma granulifera. It facilitates acetylcholine release at avian neuromuscular junctions, competes with dendrotoxin 1, a probe for voltage-dependent potassium channels, for binding to synaptosomal membranes of rat brain with a K(i) of 0.7 nM and suppresses K+ currents in rat dorsal root ganglion neurones in culture. It represents a new structural type of potassium channel toxin with the sequence V1RCDWFKETA10CRHAKSLGNC20RTSQKYRANC30AKTLQCC37 (M(r) 4275, three disulfides).

引用

页码：86 / 92

页数：7

共 37 条

[1]

BENISHIN CG, 1988, MOL PHARMACOL, V34, P152

[2] 2 POTENT CENTRAL CONVULSANT PEPTIDES, A BEE VENOM TOXIN, THE MCD PEPTIDE, AND A SNAKE-VENOM TOXIN, DENDROTOXIN-I, KNOWN TO BLOCK K+ CHANNELS, HAVE INTERACTING RECEPTOR-SITES [J].