INFLUENCE OF INCREASING METHANOL CONCENTRATIONS ON ALCOHOL OXIDASE ACTIVITY IN CANDIDA-BOIDINII

被引：4

作者：

ANDEROVA, M

ANDERA, L

VOLFOVA, O

PANOS, J

LOBL, V

机构：

[1] Institute of Microbiology, Academy of Sciences of the Czech Republic, Prague

来源：

FOLIA MICROBIOLOGICA | 1993年 / 38卷 / 01期

关键词：

D O I：

10.1007/BF02814550

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

In methanol-limited chemostat cultures methanol concentration > K(I) for growth decreased sharply the alcohol oxidase activity in yeast cells. This effect was accompanied by accumulation of riboflavin phosphate in the medium. Purified alcohol oxidase showed higher K(m) for methanol, change in absorbance maxima in the riboflavin area and chemical modification of enzyme structure discovered by means of partial proteolysis.

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页码：55 / 58

页数：4

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