A SYNTHETIC PEPTIDE OF THE N-TERMINUS OF ADP-RIBOSYLATION FACTOR (ARF) INHIBITS REGULATED EXOCYTOSIS IN ADRENAL CHROMAFFIN CELLS

被引：26

作者：

MORGAN, A ^{[1
]}

BURGOYNE, RD ^{[1
]}

机构：

[1] UNIV LIVERPOOL,PHYSIOL LAB,POB 147,LIVERPOOL L69 3BX,ENGLAND

来源：

FEBS LETTERS | 1993年 / 329卷 / 1-2期

基金：

英国惠康基金;

关键词：

EXOCYTOSIS; CALCIUM; ADP-RIBOSYLATION FACTOR; GTP-BINDING PROTEIN; CHROMAFFIN CELL;

D O I：

10.1016/0014-5793(93)80206-A

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have investigated the role of ADP-ribosylation factor (ARF) in regulated exocytosis in digitonin-permeabilized adrenal chromaffin cells by the use of a synthetic peptide, hARF1(2-17), based on the N-terminus of the protein. hARF1(2-17) inhibited Ca2+-dependent but not basal exocytosis, whereas equimolar levels of other synthetic peptides were ineffective. The inhibitory effect of hARF1(2-17) was dose-dependent and half-maximal at 12 muM. GTPgammaS-induced secretion in the presence of non-stimulatory CA2+ concentrations was also inhibited by hARF1(2-17). These results point to a hitherto unsuspected role for ARF in regulated exocytosis, and the potency of the hARF1(2-17) peptide suggests that ARF is essential for exocytosis in bovine adrenal chromaffin cells.

引用

页码：121 / 124

页数：4

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