THE EXPRESSION OF INSULIN-LIKE GROWTH-FACTORS AND THEIR BINDING-PROTEINS IN NORMAL HUMAN-LYMPHOCYTES

被引:59
作者
NYMAN, T
PEKONEN, F
机构
[1] Medix Research Unit, Tukholmankatu 2
来源
ACTA ENDOCRINOLOGICA | 1993年 / 128卷 / 02期
关键词
D O I
10.1530/acta.0.1280168
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
The expression of insulin-like growth factors and their binding proteins in normal human peripheral lymphocytes was studied using the reverse transcriptase polymerase chain reaction method and Western ligand blotting. A quantitation of RT-PCR products was used to study the differences between normal and PHA stimulated lymphocytes. Normal freshly collected lymphocytes expressed mRNAs for both IGF-I receptor and IGF-II receptor but no expression of the corresponding growth factors was detectable. After stimulation with phytohemagglutinin the lymphocytes, however, expressed both IGF-I and IGF-II. Of the five IGFBPs examined, unstimulated lymphocytes expressed only IGFBP-2 and -3. Stimulated lymphocytes expressed IGFBP-4 and -5, in addition to IGFBP-2 and -3, whereas IGFBP-1 mRNA remained undetectable. The ligand blotting of lymphocyte conditioned media revealed production of 34K, 43K and 49K IGFBPs. The addition of estrogen, progesterone, IGF-I or growth hormone did not affect secretion of IGFBPs by lymphocytes.
引用
收藏
页码:168 / 172
页数:5
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