A SENSITIVE RADIOCHEMICAL ASSAY FOR CHOLINE ACETYLASE

被引：11

作者：

DIAMOND, I

KENNEDY, EP

机构：

[1] Department of Biological Chemistry, Harvard Medical School, Boston

来源：

ANALYTICAL BIOCHEMISTRY | 1968年 / 24卷 / 01期

关键词：

D O I：

10.1016/0003-2697(68)90062-6

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A sensitive radiochemical assay for choline acetylase has been devised based on the formation of acetylcholine from tritium-labeled acetate. The acetylcholine is separated from other labeled components by chromatography on a short column of Amberlite CG-50 ion exchanger. The procedure is sufficiently specific and sensitive so that it can be used for the measurement of the acetylase in crude, unfractionated homogenates of brain. © 1968.

引用

页码：90 / &

共 6 条

[1]

CAMPAGNARI F, 1963, J BIOL CHEM, V238, P1628

[2] A RADIOCHEMICAL METHOD FOR ESTIMATION OF CHOLINE ACETYLTRANSFERASE [J].