SEPARATION AND IDENTIFICATION OF 14C-LABELED GLUCOGENIC COMPOUNDS BY PAPER CHROMATOGRAPHY

Two different two-dimensional decending paper chromatographic systems are presented which permit the simultaneous separation of the following: alanine, phenylalanine, tyrosine, glutamic, glucose, fructose, pyruvic, lactic, malic, fumaric, succinic, α-ketoglutaric, 3-phosphoglyceric, hexose phosphate, and phosphoenolpyruvic. Fructose diphosphate and 2-phosphoglyceric migrate together but can be well separated from the others. Carbon-14 labeled standards were used and localization accomplished by radioautography. The chromatography described can be applied usefully to deproteinated subcellular or tissue extracts from experiments in which isotopically labeled precursors were metabolized by the pathways of glycolysis, respiration, gluconeogenesis, or the biosynthesis and degradation of some amino acids. This is so chiefly because the discrete isolations obtained with permit the accurate determination of specific activities. © 1969.