DETERMINATION OF THIOL-CONTAINING COMPOUNDS BY SELECTIVE FLUORESCENCE-DERIVATIZATION AND HPLC

被引：37

作者：

LANKMAYR, EP ^{[1
]}

BUDNA, KW ^{[1
]}

MULLER, K ^{[1
]}

机构：

[1] BIOCHEM GMBH,A-6250 KUNDL,AUSTRIA

来源：

FRESENIUS ZEITSCHRIFT FUR ANALYTISCHE CHEMIE | 1979年 / 295卷 / 05期

关键词：

D O I：

10.1007/BF00539706

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A fast and sensitive method for the determination of thiol-containing compounds by formation of fluorescent derivatives and high pressure liquid chromatography (HPLC) is described. The conditions for the selective reaction of thiol compounds with 5-dimethylaminonaphthalene-1-sulfonylaziridine were investigated. The derivatives are stable and give high fluorescence yields. After HPLC separations a fluorescence detector was used to achieve maximum sensitivity. The detection limits for thiol containing amino acids are in the pmol range. © 1979 Springer-Verlag.

引用

页码：371 / 374

页数：4

共 7 条

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