CONSTRUCTION OF A MOUSE YEAST ARTIFICIAL CHROMOSOME LIBRARY IN A RECOMBINATION-DEFICIENT STRAIN OF YEAST

被引：78

作者：

CHARTIER, FL ^{[1
]}

KEER, JT ^{[1
]}

SUTCLIFFE, MJ ^{[1
]}

HENRIQUES, DA ^{[1
]}

MILEHAM, P ^{[1
]}

BROWN, SDM ^{[1
]}

机构：

[1] ST MARYS HOSP,SCH MED,DEPT BIOCHEM & MOLEC GENET,NORFOLK PL,LONDON W2 1PG,ENGLAND

来源：

NATURE GENETICS | 1992年 / 1卷 / 02期

关键词：

D O I：

10.1038/ng0592-132

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

We have constructed a new generation yeast artificial chromosome (YAC) library from female C57BL/10 mice in a recombination-deficient strain of Saccharomyces cerevisiae carrying a mutation in the RAD52 gene. The YAC library contains 41,568 clones with an average insert size of 240 kilobases, representing a greater than threefold coverage of the mouse genome. Currently, the library can be screened by polymerase chain reaction and we have isolated positive clones at a number of loci in the mouse genome. This rad52 library should enable a long-term assessment of the effect of one of the yeast recombination pathway genes on both, genome-wide YAC clone stability and the frequency of chimaeric clones.

引用

页码：132 / 136

页数：5

共 27 条

[1] CONSTRUCTION AND CHARACTERIZATION OF A YEAST ARTIFICIAL CHROMOSOME LIBRARY CONTAINING 7 HAPLOID HUMAN GENOME EQUIVALENTS
ALBERTSEN, HM
ABDERRAHIM, H
CANN, HM
DAUSSET, J
LEPASLIER, D
COHEN, D
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (11) : 4256 - 4260
[2] A 3.5 GENOME EQUIVALENT MULTIACCESS YAC LIBRARY - CONSTRUCTION, CHARACTERIZATION, SCREENING AND STORAGE
ANAND, R
RILEY, JH
BUTLER, R
SMITH, JC
MARKHAM, AF
[J]. NUCLEIC ACIDS RESEARCH, 1990, 18 (08) : 1951 - 1956
[3] HIGH-DENSITY MOLECULAR MAP OF THE CENTRAL SPAN OF THE MOUSE X-CHROMOSOME
BROCKDORFF, N
KAY, G
SMITH, S
KEER, JT
HAMVAS, RMJ
BROWN, SDM
RASTAN, S
[J]. GENOMICS, 1991, 10 (01) : 17 - 22
[4] ISOLATION OF SINGLE-COPY HUMAN GENES FROM A LIBRARY OF YEAST ARTIFICIAL CHROMOSOME CLONES
BROWNSTEIN, BH
SILVERMAN, GA
LITTLE, RD
BURKE, DT
KORSMEYER, SJ
SCHLESSINGER, D
OLSON, MV
[J]. SCIENCE, 1989, 244 (4910) : 1348 - 1351
[5] TRANSFORMATION OF YEAST SPHEROPLASTS WITHOUT CELL-FUSION
BURGERS, PMJ
PERCIVAL, KJ
[J]. ANALYTICAL BIOCHEMISTRY, 1987, 163 (02) : 391 - 397
[6] CLONING OF LARGE SEGMENTS OF EXOGENOUS DNA INTO YEAST BY MEANS OF ARTIFICIAL CHROMOSOME VECTORS
BURKE, DT
CARLE, GF
OLSON, MV
[J]. SCIENCE, 1987, 236 (4803) : 806 - 812
[7] BURKE DT, 1991, MAMM GENOME, V65
[8] ESTABLISHMENT OF THE MOUSE CHROMOSOME-7 REGION WITH HOMOLOGY TO THE MYOTONIC-DYSTROPHY REGION OF HUMAN CHROMOSOME-19Q
CAVANNA, JS
GREENFIELD, AJ
JOHNSON, KJ
MARKS, AR
NADALGINARD, B
BROWN, SDM
[J]. GENOMICS, 1990, 7 (01) : 12 - 18
[9] YEAST GENE RAD52 CAN SUBSTITUTE FOR PHAGE-T4 GENE-46 OR GENE-47 IN CARRYING OUT RECOMBINATION AND DNA-REPAIR
CHEN, DS
BERNSTEIN, H
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (18) : 6821 - 6825
[10] CLARK GJ, 1992, IMMUNOGENETICS, V35, P54

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