INTERACTION BETWEEN MICROVASCULAR ALPHA-1-ADRENOCEPTORS AND ALPHA-2-ADRENOCEPTORS AND ENDOTHELIUM-DERIVED RELAXING FACTOR

Intravital microscopy was used to study the effect of endothelium-derived relaxing factor (EDRF) on microvascular adrenoceptor sensitivity in rat cremaster skeletal muscle. N(G)-Monomethyl L-arginine (L-NMMA, 1-300-mu-M), an inhibitor of EDRF formation, produced concentration-dependent constriction of arterioles and venules. When an intermediate amount of alpha-1- versus alpha-2-adrenoceptor tone was first produced with bath-added norepinephrine (NE) in the presence of rauwolscine or prazosin, L-NMMA caused constriction with greater potency and efficacy during alpha-2 than during alpha-1 tone. During localized alpha-1 or alpha-2 constriction along an arteriole by perivascular micropipette suffusion of NE in the presence of rauwolscine or prazosin, again, bath-added L-NMMA produced constriction with greater potency during alpha-2 than during alpha-1 constriction. Like L-NMMA, disruption of EDRF release by microembolization caused baseline arteriole constriction and selectively increased alpha-2 sensitivity 75-fold. Although these findings support the hypothesis that endothelial cells possess alpha-2-adrenoceptors that promote EDRF release, a greater susceptibility of alpha-2 than alpha-1 constriction to EDRF inhibition could also account for the results. In support of this latter possibility, alpha-2 constriction was approximately 50-fold more susceptible than alpha-1 constriction to inhibition by the EDRF-like nitrodilator nitroprusside. The similarity in magnitude of this difference in sensitivity with the difference obtained in the embolization experiments does not support the hypothesis that microvascular endothelial cells in skeletal muscle possess EDRF-promoting alpha-2-adrenoceptors. However, these data do suggest that endogenous EDRF release modulates basal arteriole and venule tone and that alpha-2-adrenoceptor constriction is more sensitive than alpha-1 constriction to inhibition by EDRF.