FOLDING INVITRO OF BOVINE PANCREATIC TRYPSIN-INHIBITOR IN THE PRESENCE OF PROTEINS OF THE ENDOPLASMIC-RETICULUM

被引：62

作者：

ZAPUN, A

CREIGHTON, TE

ROWLING, PJE

FREEDMAN, RB

机构：

[1] EUROPEAN MOLEC BIOL LAB, MEYERHOFSTR 1, W-6900 HEIDELBERG, GERMANY

[2] UNIV KENT, BIOL LAB, CANTERBURY CT2 7NJ, ENGLAND

[3] MRC, MOLEC BIOL LAB, CAMBRIDGE CB2 2QH, ENGLAND

来源：

PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS | 1992年 / 14卷 / 01期

关键词：

PROTEIN DISULFIDE ISOMERASE; DISULFIDE BONDS; PROTEIN FOLDING; CHAPERONES;

D O I：

10.1002/prot.340140104

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The rates of folding and disulfide bond formation in reduced BPTI were measured in vitro in the presence and absence of total protein from the endoplasmic reticulum. The rates were increased substantially by the endoplasmic reticulum proteins, but only to the extent expected from the known content and activity of protein-disulfide-isomerase. No effects of added ATP or Ca2+ were observed, even though protein-disulfide-isomerase binds Ca2+ tightly.

引用

收藏

页码：10 / 15

页数：6

相关论文

共 25 条

[1] TRANSIENT ASSOCIATION OF NEWLY SYNTHESIZED UNFOLDED PROTEINS WITH THE HEAT-SHOCK GROEL PROTEIN [J].

BOCHKAREVA, ES ;

LISSIN, NM ;

GIRSHOVICH, AS .

NATURE, 1988, 336 (6196) :254-257

[2]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[3] DEFECTIVE CO-TRANSLATIONAL FORMATION OF DISULFIDE BONDS IN PROTEIN DISULFIDE-ISOMERASE-DEFICIENT MICROSOMES [J].

BULLEID, NJ ;

FREEDMAN, RB .

NATURE, 1988, 335 (6191) :649-651

[4] ASSEMBLY OF INFLUENZA HEMAGGLUTININ TRIMERS AND ITS ROLE IN INTRACELLULAR-TRANSPORT [J].

COPELAND, CS ;

DOMS, RW ;

BOLZAU, EM ;

WEBSTER, RG ;

HELENIUS, A .

JOURNAL OF CELL BIOLOGY, 1986, 103 (04) :1179-1191

[5] TOWARD A BETTER UNDERSTANDING OF PROTEIN FOLDING PATHWAYS [J].

CREIGHTON, TE .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (14) :5082-5086

[6] CATALYSIS BY PROTEIN-DISULFIDE ISOMERASE OF THE UNFOLDING AND REFOLDING OF PROTEINS WITH DISULFIDE BONDS [J].

CREIGHTON, TE ;

HILLSON, DA ;

FREEDMAN, RB .

JOURNAL OF MOLECULAR BIOLOGY, 1980, 142 (01) :43-62

[7] SEQUENCES OF THE GENES AND POLYPEPTIDE PRECURSORS FOR 2 BOVINE PROTEASE INHIBITORS [J].

CREIGHTON, TE ;

CHARLES, IG .

JOURNAL OF MOLECULAR BIOLOGY, 1987, 194 (01) :11-22

[8] DISULFIDE BONDS AND PROTEIN STABILITY [J].

CREIGHTON, TE .

BIOESSAYS, 1988, 8 (2-3) :57-63

[9] KINETIC ROLE OF A METASTABLE NATIVE-LIKE 2-DISULFIDE SPECIES IN THE FOLDING TRANSITION OF BOVINE PANCREATIC TRYPSIN-INHIBITOR [J].

CREIGHTON, TE ;

GOLDENBERG, DP .

JOURNAL OF MOLECULAR BIOLOGY, 1984, 179 (03) :497-526

[10] PEPTIDE BINDING AND RELEASE BY PROTEINS IMPLICATED AS CATALYSTS OF PROTEIN ASSEMBLY [J].

FLYNN, GC ;

CHAPPELL, TG ;

ROTHMAN, JE .

SCIENCE, 1989, 245 (4916) :385-390