HUMAN PERIODONTAL CELLS INITIATE MINERAL-LIKE NODULES INVITRO

被引:180
作者
ARCEO, N
SAUK, JJ
MOEHRING, J
FOSTER, RA
SOMERMAN, MJ
机构
[1] UNIV MARYLAND,SCH DENT,DEPT PATHOL,666 W BALTIMORE ST,ROOM 4-A-28,BALTIMORE,MD 21201
[2] UNIV MARYLAND,SCH DENT,DEPT PERIODONT,BALTIMORE,MD 21201
[3] UNIV MARYLAND,SCH DENT,DEPT PERIODONTOL,BALTIMORE,MD 21201
关键词
PERIODONTAL ATTACHMENT; CONNECTIVE TISSUE; FIBROBLASTS; CELLS; PERIODONTAL LIGAMENT; OSTEOBLAST-LIKE;
D O I
10.1902/jop.1991.62.8.499
中图分类号
R78 [口腔科学];
学科分类号
1003 ;
摘要
A PRIMARY OBJECTIVE IN THE TREATMENT of periodontal diseases is the regeneration of the mineralized and soft connective tissue components of the attachment apparatus. Current theories suggest the cells of the periodontium have the capacity, when appropriately triggered, to actively participate in restoring connective tissues, including mineralized tissues. To evaluate further the role of such cells in periodontal homeostasis, periodontal ligament (PDL) cells and gingival fibroblasts (GF) were cultured and examined for alkaline phosphatase levels and for the ability to produce mineralized nodules in culture. These are two characteristics of osteoblast-like cells in vitro. The levels of alkaline phosphatase produced by these cells were determined by a modified kinetic assay and the ability of these cells to produce mineral-like nodules in vitro was evaluated by Von Kossa staining and light and electron microscopy. PDL cells had significantly higher levels of alkaline phosphatase when compared with gingival fibroblasts obtained from the same patient and the same passage, in vitro. Furthermore PDL cells, but not GF, were capable of producing mineral-like nodules in vitro. These results indicate differences in behavior between PDL cells and GF; such differences may prove important in designing appropriate clinical therapies directed at stimulating periodontal regeneration.
引用
收藏
页码:499 / 503
页数:5
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