DEVELOPMENT OF A PCR FOR IDENTIFICATION OF NAEGLERIA-FOWLERI FROM THE ENVIRONMENT

被引：28

作者：

KILVINGTON, S ^{[1
]}

BEECHING, J ^{[1
]}

机构：

[1] UNIV BATH,SCH BIOL & BIOCHEM,BATH BA2 7AY,AVON,ENGLAND

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 1995年 / 61卷 / 10期

关键词：

D O I：

10.1128/AEM.61.10.3764-3767.1995

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A species-specific PCR for the identification of Naegleria fowleri was developed. In sensitivity studies, 10 trophozoites or cysts and 1 trophozoite or cyst could be detected after 35 and 45 cycles, respectively. In conjunction with a rapid DNA isolation method, this PCR was used to identify N. fowleri directly from primary cultures of environmental samples.

引用

页码：3764 / 3767

页数：4

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