ASPARTATE-AMINOTRANSFERASE ACTIVITY IN MOTILE AND IMMOTILE SPERMATOZOA FRACTIONS OF FROZEN-THAWED BOAR SEMEN OBTAINED AFTER FILTRATION ON COLUMNS FILLED WITH CHITIN

被引：17

作者：

CIERESZKO, A

JABLONOWSKA, C

STRZEZEK, J

机构：

[1] Department of Animal Biochemistry, University of Agriculture and Technology

来源：

ANIMAL REPRODUCTION SCIENCE | 1990年 / 23卷 / 03期

关键词：

D O I：

10.1016/0378-4320(90)90006-2

中图分类号：

S8 [畜牧、动物医学、狩猎、蚕、蜂];

学科分类号：

0905 ;

摘要：

Frozen-thawed boar semen was separated on columns filled with chitin. With this method, fractions of motile and immotile spermatozoa were obtained. Aspartate aminotransferase (AAT) activity was determined in supernatants obtained after cold shock of motile and immotile spermatozoa. AAT activity was five times higher in motile than in immotile cells. Indirect activity of the enzyme was also observed, liberated from spermatozoa before separation. AAT activity correlated significantly with sperm concentration in all semen fractions. The coefficient of correlation between the enzyme activity (expressed per 109 spermatozoa) and sperm motility after chromatographic separation was 0.71, which was highly significant. © 1990.

引用

页码：237 / 244

页数：8

共 31 条

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