REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC APPROACH TO DETERMINE TOTAL LYMPHOCYTE CONCENTRATIONS OF 6-THIOGUANINE, METHYLMERCAPTOPURINE AND METHYLTHIOGUANINE IN HUMANS

被引：20

作者：

ERDMANN, GR ^{[1
]}

STEURY, JC ^{[1
]}

CARLETON, BC ^{[1
]}

STAFFORD, RJ ^{[1
]}

BOSTROM, BC ^{[1
]}

CANAFAX, DM ^{[1
]}

机构：

[1] MINNEAPOLIS CHILDRENS HLTH CTR,MINNEAPOLIS,MN 55404

来源：

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1991年 / 571卷 / 1-2期

关键词：

D O I：

10.1016/0378-4347(91)80441-E

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A reversed-phase high-performance liquid chromatographic (HPLC) procedure was developed to quantify intracellular lymphocyte 6-thioguanine, methylmercaptopurine and methylthioguanine. The free base of each metabolite was obtained by acid hydrolysis, which allowed for a total determination of thiopurine metabolites. 6-Thioguanine was analyzed on an octadecylsilane column using acetonitrile-10 mM sodium phosphate (11:89), pH 7, containing 0.06% tetrabutylammonium chloride. 6-Thioguanine was oxidized with potassium permanganate, and fluorescence was measured at 330 nm excitation and 410 nm emission. Methylmercaptopurine and methylthioguanine were separated on a cyanopropylsilane column using methanol-40 mM sodium phosphate (22:78), pH 2.7, and detected by ultraviolet absorbance at 314 and 290 nm, respectively.

引用

页码：149 / 156

页数：8

共 10 条

[1] QUANTITATIVE-DETERMINATION OF METABOLITES OF 6-MERCAPTOPURINE IN BIOLOGICAL-MATERIALS .3. DETERMINATION OF C-14-LABELED 6-THIOPURINES IN L5178Y CELL-EXTRACTS USING HIGH-PRESSURE LIQUID CATION-EXCHANGE CHROMATOGRAPHY [J].