PRODUCTION AND PROPERTIES OF AN ALKALINE PROTEASE BY AUREOBASIDIUM-PULLULANS

被引:49
作者
DONAGHY, JA
MCKAY, AM
机构
[1] QUEENS UNIV BELFAST,DEPT FOOD MICROBIOL,NEWFORGE LANE,BELFAST BT9 5PX,ANTRIM,NORTH IRELAND
[2] NO IRELAND DEPT AGR,DIV FOOD & AGR MICROBIOL RES,BELFAST BT4 3SB,ANTRIM,NORTH IRELAND
来源
JOURNAL OF APPLIED BACTERIOLOGY | 1993年 / 74卷 / 06期
关键词
D O I
10.1111/j.1365-2672.1993.tb05200.x
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
A strain of the yeast-like fungus Aureobasidium pullulans was grown on whey to produce an extracellular protease. The protease was totally inhibited by the serine inhibitor, phenyl methyl sulphonyl fluoride (PMSF), and partially inhibited by the chelating agent EDTA. The enzyme showed maximal activity in the alkaline range with an optimum pH of 9.5-10.5. The optimum temperature for protease activity was 41-degrees-C. As well as being active against the non-specific proteolytic substrate Azocoll, the protease readily degraded purified alpha-casein. A molecular weight of 27000 +/- 350 was determined for the protease using gel filtration chromatography.
引用
收藏
页码:662 / 666
页数:5
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