FORMATION OF MICROSOMAL CYTOCHROME-P-450 COMPLEXES STUDIED BY NMR RELAXATION OF WATER

被引：8

作者：

GRASDALEN, H ^{[1
]}

ERIKSSON, LEG ^{[1
]}

EHRENBERG, A ^{[1
]}

BACKSTROM, D ^{[1
]}

机构：

[1] UNIV STOCKHOLM,ARRHENIUS LAB,DEPT BIOPHYS,S-10691 STOCKHOLM,SWEDEN

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1978年 / 541卷 / 04期

基金：

英国医学研究理事会;

关键词：

D O I：

10.1016/0304-4165(78)90161-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Cytochrome P-450 in microsomes from liver of phenobarbital treated and control rats has been studied by light absorption and by magnetic resonance methods (EPR and NMR). The nuclear relaxation rate of water protons was measured for microsomal suspensions in the presence of various reactants of Type I and II. The change of relaxation rates correlates well with the spin state conversion of the heme iron. No competition between eventual inner-sphere water molecules and the reactants seems to occur. The temperature dependence of the low spin to high spin equilibrium was studied by light absorption and was accounted for in the temperature variation of the molar relaxation rates of the two spin states. © 1978.

引用

页码：521 / 527

页数：7

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