RATE OF SEGREGATION DUE TO PLASMID INCOMPATIBILITY

We calculated the rates of segregation due to plasmid incompatibility under several simple models. A common feature of all the models that we considered is that incompatibility is caused by the inability of the segregation mechanism to distinguish between two incompatible plasmids. We measured the rate of segregation due to incompatibility of a pair of ColE1 derivatives under two conditions: (1) One plasmid was introduced into cells carrying the other by conjugation. (2) Cells carrying both plasmids were maintained by selection and then selection was released. Interpretation of the results was made more difficult by effects of the Plasmids on the host cell's growth rate. These experiments gave results in agreement with the predictions of a random pool replication model. Published results were also in reasonable agreement with this model. © 1979, Cambridge University Press. All rights reserved.