EFFECT OF MOBILE PHASE DENSITY GRADIENTS ON EFFICIENCY IN PACKED-COLUMN SUPERCRITICAL FLUID CHROMATOGRAPHY

被引：29

作者：

BERGER, TA

DEYE, JF

机构：

[1] Hewlett-Packard Company, Avondale, 19311-0900, PA

来源：

CHROMATOGRAPHIA | 1990年 / 30卷 / 1-2期

关键词：

Density gradients; Effect on efficiency; Packed columns; Supercritical fluid chromatography; (SFC);

D O I：

10.1007/BF02270449

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The efficiency of packed columns was measured as a function of flow rate, temperature, outlet density, and the density differential across the column, unsing pure carbon dioxide as the mobile phase. Although density differentials are often blamed for a serious loss in efficiency in packed column supercritical fluid chromatography, the results show that efficiency was not a function of the density differential. Peak shapes suggest that apparent loss in efficiency is actually due to inadequate solubility of the solute in carbon dioxide. © 1990 Friedr. Vieweg & Sohn Verlagsgesellschaft mbH.

引用

页码：57 / 60

页数：4

共 15 条

[1] BENDER E, 1970, 5TH P S THERM PROP N
[2] SUPERCRITICAL FLUID CHROMATOGRAPHY WITH SMALL PARTICLE DIAMETER PACKED-COLUMNS
GERE, DR
BOARD, R
MCMANIGILL, D
[J]. ANALYTICAL CHEMISTRY, 1982, 54 (04) : 736 - 740
[3] EFFECTS OF COLUMN LENGTH, PARTICLE-SIZE, FLOW-RATE, AND PRESSURE PROGRAMMING RATE ON RESOLUTION IN PRESSURE-PROGRAMMED SUPERCRITICAL FLUID CHROMATOGRAPHY
GRAHAM, JA
ROGERS, LB
[J]. JOURNAL OF CHROMATOGRAPHIC SCIENCE, 1980, 18 (02) : 75 - 84
[4] ISOCRATIC NETWORKS IN SUPERCRITICAL FLUID CHROMATOGRAPHY .2. SELECTIVITIES AND EFFECTIVE PLATE NUMBERS
LEYENDECKER, D
LEYENDECKER, D
SCHMITZ, FP
KLESPER, E
[J]. JOURNAL OF HIGH RESOLUTION CHROMATOGRAPHY & CHROMATOGRAPHY COMMUNICATIONS, 1987, 10 (03): : 141 - 144
[5] SUPERCRITICAL FLUID CHROMATOGRAPHY USING MIXTURES OF CARBON-DIOXIDE OR ETHANE WITH 1,4-DIOXANE AS ELUENTS
LEYENDECKER, D
LEYENDECKER, D
SCHMITZ, FP
LORENSCHAT, B
KLESPER, E
[J]. JOURNAL OF CHROMATOGRAPHY, 1987, 398 : 105 - 123
[6] 3-DIMENSIONAL NETWORK PLOTS IN SUPERCRITICAL FLUID CHROMATOGRAPHY WITH NORMAL-PENTANE-1,4-DIOXANE
LEYENDECKER, D
SCHMITZ, FP
LEYENDECKER, D
KLESPER, E
[J]. JOURNAL OF CHROMATOGRAPHY, 1987, 393 (02): : 155 - 167
[7] CHROMATOGRAPHY WITH SUBCRITICAL AND SUPERCRITICAL ELUENTS - INFLUENCE OF TEMPERATURE, PRESSURE AND FLOW-RATE ON THE BEHAVIOR OF LOWER ALKANES
LEYENDECKER, D
SCHMITZ, FP
LEYENDECKER, D
KLESPER, E
[J]. JOURNAL OF CHROMATOGRAPHY, 1985, 321 (02): : 273 - 286
[8] INFLUENCES OF TEMPERATURE, PRESSURE AND ELUENT COMPOSITION ON THE BEHAVIOR OF BINARY ELUENTS AT SUBCRITICAL AND SUPERCRITICAL CONDITIONS - PENTANE AND DIETHYLENEGLYCOL DIMETHYL ETHER
LEYENDECKER, D
SCHMITZ, FP
KLESPER, E
[J]. CHROMATOGRAPHIA, 1987, 23 (03) : 171 - 178
[9] CHROMATOGRAPHY WITH SUBCRITICAL AND SUPERCRITICAL ELUENTS - THE INFLUENCE OF TEMPERATURE, PRESSURE AND FLOW-RATE ON THE BEHAVIOR OF DIMETHYL AND DIETHYL-ETHER
LEYENDECKER, D
SCHMITZ, FP
KLESPER, E
[J]. JOURNAL OF CHROMATOGRAPHY, 1984, 315 (DEC): : 19 - 30
[10] THE DEPENDENCE OF REDUCED PLATE HEIGHT ON REDUCED VELOCITY IN CARBON-DIOXIDE SUPERCRITICAL FLUID CHROMATOGRAPHY WITH PACKED-COLUMNS
MOURIER, PA
CAUDE, MH
ROSSET, RH
[J]. CHROMATOGRAPHIA, 1987, 23 (01) : 21 - 25

← 1 2 →