NONAQUEOUS ION-EXCHANGE SEPARATION TECHNIQUE FOR USE IN BIOASSAY-DIRECTED FRACTIONATION OF COMPLEX-MIXTURES - APPLICATION TO WOOD SMOKE PARTICLE EXTRACTS

被引：14

作者：

BELL, DA ^{[1
]}

KARAM, H ^{[1
]}

KAMENS, RM ^{[1
]}

机构：

[1] UNIV N CAROLINA,SCH PUBL HLTH,DEPT ENVIRONM SCI & ENGN,CHAPEL HILL,NC 27514

来源：

ENVIRONMENTAL SCIENCE & TECHNOLOGY | 1990年 / 24卷 / 08期

关键词：

D O I：

10.1021/es00078a016

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

We have explored the feasibility of an alternative method for acid/base/neutral separation of atmospheric samples such as wood smoke particle extracts that contain highly polar or acidic organic species. Ion-exchange resins, Amberlyst 15 and Amberlyst 26, were used with organic solvents for fractionating a standard mixture of organic acid, base, and neutral compounds, and a dichloromethane extract from pine wood smoke particles. Total recovery of individual standard compounds was 85-124% and qualitative separation between chemical classes was good. The recovery of wood soot extract through the fractionation system was 107 ± 13%, with 15% in the basic fraction, 55% in the neutral fraction, and 20% and 17% in two acidic fractions. The majority of bacterial mutagenicity (TA98+S9, 49%; TA98-S9, 61%) appeared in the neutral fraction but significant direct-acting mutagenicity (39%) was found in the acidic fractions. © 1990, American Chemical Society. All rights reserved.

引用

页码：1261 / 1264

页数：4

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