ESTIMATION OF TYROSINE BY THE ATTENUATION OF 4-IODOPHENOL ENHANCED CHEMILUMINESCENCE

被引：10

作者：

CANDY, TEG

MANTLE, D

JONES, P

机构：

[1] UNIV NEWCASTLE UPON TYNE,DEPT CHEM,BEDSON BLDG,NEWCASTLE TYNE NE1 7RU,TYNE & WEAR,ENGLAND

[2] NEWCASTLE GEN HOSP,DEPT NEUROCHEM,NEWCASTLE TYNE NE4 6BE,TYNE & WEAR,ENGLAND

来源：

JOURNAL OF BIOLUMINESCENCE AND CHEMILUMINESCENCE | 1991年 / 6卷 / 04期

关键词：

ENHANCED CHEMILUMINESCENCE; TYROSINE ANALYSIS; PROTEIN CATABOLISM;

D O I：

10.1002/bio.1170060406

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Tyrosine markedly attenuates the chemiluminescence output intensity from the 4-iodophenol enhanced chemiluminescence assay system in a manner consistent with competition between the amino acid and luminol for the 4-iodophenoxy radical. This effect provides the basis for a sensitive assay of tyrosine. Interference by the other amino acids has been assessed; major interference by cysteine can be removed by incubation with iodoacetic acid.

引用

页码：245 / 249

页数：5

共 11 条

[1] SPECTROPHOTOMETRIC STUDY OF THE REACTION OF PROTEIN SULFHYDRYL GROUPS WITH ORGANIC MERCURIALS [J].