NONDISSOCIATION OF GAL4 AND GAL80 INVIVO AFTER GALACTOSE INDUCTION

被引：127

作者：

LEUTHER, KK

JOHNSTON, SA

机构：

[1] UNIV TEXAS,SW MED CTR,DEPT MED,DALLAS,TX 75235

[2] UNIV TEXAS,SW MED CTR,DEPT BIOCHEM,DALLAS,TX 75235

来源：

SCIENCE | 1992年 / 256卷 / 5061期

关键词：

D O I：

10.1126/science.1598579

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Transcription of galactose-inducible genes in yeast is regulated by interaction between the activator protein GAL4 and the negative regulatory protein GAL80. It has been suggested that GAL80 binds to and represses GAL4 under uninduced conditions and dissociates from GAL4 on induction. However, the possibility that GAL80 remains associated with GAL4 after induction has not been ruled out. Experiments to discriminate between these two models were performed and revealed that GAL80 stays bound after induction.

引用

页码：1333 / 1335

页数：3

共 14 条

[1] ACTIVATION OF YEAST POLYMERASE-II TRANSCRIPTION BY HERPESVIRUS VP16 AND GAL4 DERIVATIVES INVITRO
CHASMAN, DI
LEATHERWOOD, J
CAREY, M
PTASHNE, M
KORNBERG, RD
[J]. MOLECULAR AND CELLULAR BIOLOGY, 1989, 9 (11) : 4746 - 4749
[2] SPECIFIC DNA-BINDING OF GAL4, A POSITIVE REGULATORY PROTEIN OF YEAST
GINIGER, E
VARNUM, SM
PTASHNE, M
[J]. CELL, 1985, 40 (04) : 767 - 774
[3] ISOLATION OF THE YEAST REGULATORY GENE GAL4 AND ANALYSIS OF ITS DOSAGE EFFECTS ON THE GALACTOSE MELIBIOSE REGULON
JOHNSTON, SA
HOPPER, JE
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1982, 79 (22): : 6971 - 6975
[4] LANGDON SD, UNPUB
[5] LEUTHER KK, UNPUB
[6] INTERACTION OF GAL4 AND GAL80 GENE REGULATORY PROTEINS INVITRO
LUE, NF
CHASMAN, DI
BUCHMAN, AR
KORNBERG, RD
[J]. MOLECULAR AND CELLULAR BIOLOGY, 1987, 7 (10) : 3446 - 3451
[7] CONVERTING A EUKARYOTIC TRANSCRIPTIONAL INHIBITOR INTO AN ACTIVATOR
MA, J
PTASHNE, M
[J]. CELL, 1988, 55 (03) : 443 - 446
[8] THE CARBOXY-TERMINAL 30 AMINO-ACIDS OF GAL4 ARE RECOGNIZED BY GAL80
MA, J
PTASHNE, M
[J]. CELL, 1987, 50 (01) : 137 - 142
[9] MATSUMOTO K, 1980, J BACTERIOL, V141, P509
[10] PARTHUN MR, 1990, J BIOL CHEM, V265, P209

← 1 2 →