UPTAKE OF THE RESTRICTION ENDONUCLEASE ALUI BY CHINESE-HAMSTER OVARY CELLS MEASURED BY FREQUENCIES OF INDUCED CHROMOSOMAL-ABERRATIONS - EFFECT OF HYPERTONIC CONCENTRATIONS OF GLYCEROL AND SORBITOL

被引：16

作者：

JOHANNES, C

BOES, R

OBE, G

机构：

[1] University GH Essen, Department of Genetics, D-4300 Essen 1

来源：

MUTAGENESIS | 1992年 / 7卷 / 03期

关键词：

D O I：

10.1093/mutage/7.3.225

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Chinese hamster ovary cells were exposed to the restriction endonuclease AluI in the presence of 2.2 M glycerol or 0.9 M sorbitol using different treatment regimens. Induced chromosomal aberrations were taken as indication for cellular uptake of AluI. The results indicate that cellular uptake of AluI is mediated by vesicles formed during transition of hypertonic to isotonic conditions. Addition of medium leads to breakdown of these vesicles and to liberation of AluI molecules which eventually may reach the chromatin.

引用

页码：225 / 232

页数：8

共 9 条

[1]

CLEGG JS, 1986, EXP CELL RES, V163, P35, DOI 10.1016/0014-4827(86)90556-2

[2] RADIATION-INDUCED CHROMOSOME-ABERRATIONS AND THE POISSON-DISTRIBUTION [J].