VALIDATION OF A RADIOIMMUNOASSAY FOR INDOLE-3-ACETIC-ACID USING GAS CHROMATOGRAPHY-SELECTED ION MONITORING MASS-SPECTROMETRY

被引：34

作者：

PENGELLY, WL ^{[1
]}

BANDURSKI, RS ^{[1
]}

SCHULZE, A ^{[1
]}

机构：

[1] MICHIGAN STATE UNIV, DEPT BOT & PLANT PATHOL, E LANSING, MI 48824 USA

来源：

PLANT PHYSIOLOGY | 1981年 / 68卷 / 01期

关键词：

D O I：

10.1104/pp.68.1.96

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

A radioimmunoassay for IAA was validated by comparison with a physico-chemical assay utilizing gas chromatography-selected ion monitoring-mass spectrometry and 4,5,6,7-tetradeutero-IAA as an internal standard. The radioimmunoassay provided a reliable estimate of the free IAA content of etiolated corn [Zea mays cv. Stowell''s Evergreen Sweet Corn] shoots. Base hydrolysis of extracts for determination of ester IAA released substances which interfered with the radioimmunoassay. Interference was detected by internal controls and by lack of agreement with the mass spectral method. Interfering compounds could be removed from extracts by chromatography on diethylaminoethyl- and hydroxypropylated (lipophilic) Sephadex G-25. Following such purification the radioimmunoassay estimate of the total (free + ester) IAA content of etiolated corn shoots agreed with the mass spectral method within 2% on the average.

引用

页码：96 / 98

页数：3

共 4 条

[1] SYNTHESIS OF 4,5,6,7 AND 2,4,5,6,7 DEUTERIUM-LABELED INDOLE-3-ACETIC-ACID FOR USE IN MASS-SPECTROMETRIC ASSAYS
MAGNUS, V
BANDURSKI, RS
SCHULZE, A
[J]. PLANT PHYSIOLOGY, 1980, 66 (04) : 775 - 781
[2] SPECIFIC RADIOIMMUNOASSAY FOR NANOGRAM QUANTITIES OF AUXIN, INDOLE-3-ACETIC-ACID
PENGELLY, W
MEINS, F
[J]. PLANTA, 1977, 136 (02) : 173 - 180
[3] ESTERIFICATION OF FATTY ACIDS WITH DIAZOMETHANE ON A SMALL SCALE
SCHLENK, H
GELLERMAN, JL
[J]. ANALYTICAL CHEMISTRY, 1960, 32 (11) : 1412 - 1414
[4] YALOW RS, 1978, SCIENCE, V200, P1236, DOI 10.1118/1.594477

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