PHOSPHOMONOESTER IS ASSOCIATED WITH PROLIFERATION IN HUMAN BREAST-CANCER - A P-31 MRS STUDY

被引:56
作者
KALRA, R
WADE, KE
HANDS, L
STYLES, P
CAMPLEJOHN, R
GREENALL, M
ADAMS, GE
HARRIS, AL
RADDA, GK
机构
[1] MRC,RADIOBIOL UNIT,CHILTON OX11 ORD,OXON,ENGLAND
[2] JOHN RADCLIFFE HOSP,DEPT ONCOL,ICRF,OXFORD OX3 9DU,ENGLAND
[3] JOHN RADCLIFFE HOSP,DEPT SURG,OXFORD OX3 9DU,ENGLAND
[4] JOHN RADCLIFFE HOSP,MRC,BIOCHEM & CLIN MAGNET RESONANCE UNIT,OXFORD OX3 9DU,ENGLAND
[5] ST THOMAS HOSP,RICHARD DIMBLEBY DEPT CANC RES,LONDON SE1 7EH,ENGLAND
关键词
D O I
10.1038/bjc.1993.211
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Phospholipid metabolism of human breast cancer was studied by P-31 magnetic resonance spectroscopy (MRS). In vivo localised P-31 MR spectra were obtained from the tumour alone using phase modulated rotating frame imaging. For 31 tumours, median (range) phosphomonoester (PME) to ATP ratio was 1.48 (0.57-3.78) and phosphodiester (PDE) to ATP ratio was 1.65 (0.44-3.89). DNA index and S phase fraction (SPF) were measured by flow cytometry of paraffin embedded tissue. Twelve (39%) tumours were diploid and 19 aneuploid. Median (range) SPF for 29 assessable tumours was 5.3% (0.6-28%), with significantly greater median SPF for aneuploid tumours (9.3%) than diploid (3.8%, P = 0.007). There was a significant association between PME/ATP and SPF (P = 0.03) due to a significant correlation for aneuploid tumours (P = 0.0 1). High resolution P-31 MRS of extracts from.18 tumours (including seven studied in vivo) demonstrated that the PME peak consists predominantly of phosphoethanolamine (PE) with a smaller contribution from phosphocholine (PC) (median (range) PE/PC: 3.02 (1.13-5.09)). Changes in PME/ATP were observed for two tumours where tamoxifen stablised disease and may be consistent with the cytostatic effects of this drug.
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页码:1145 / 1153
页数:9
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