CROSS-LINKING AND CLEAVAGE OF PBR322 DNA PHOTOSENSITIZED BY 7-METHYLPYRIDO[3,4-C]PSORALEN

被引：11

作者：

CHEN, XS ^{[1
]}

KAGAN, J ^{[1
]}

机构：

[1] UNIV ILLINOIS,DEPT CHEM,CHICAGO,IL 60607

来源：

JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY | 1994年 / 23卷 / 01期

关键词：

DNA CLEAVAGE; DNA CROSS-LINKING; DNA DENATURATION; PHOTOSENSITIZATION; PBR322; RESTRICTION ENZYME; ENZYME;

D O I：

10.1016/1011-1344(93)06979-D

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

7-Methylpyrido[3,4-c]psoralen (7-MPP) had been designed to be a monofunctional sensitizer in which the pyrone double bond, being engaged in a pyridine ring, would be incapable of participating in the formation of a cyclobutane ring with a DNA component. However, one example of photosensitization of thymine-thymine dimer formation in DNA by 7-MPP had been reported. This paper proves that 7-MPP can sensitize interstrand crosslinks in pBR322 DNA. It also proves that 7-MPP photosensitizes double strand cleavage reactions in the DNA with an unusually large degree of site selectivity.

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页码：27 / 33

页数：7

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