EFFECTS OF ALPHA-ARBUTIN AND BETA-ARBUTIN ON ACTIVITY OF TYROSINASES FROM MUSHROOM AND MOUSE MELANOMA

被引:167
作者
FUNAYAMA, M [1 ]
ARAKAWA, H [1 ]
YAMAMOTO, R [1 ]
NISHINO, T [1 ]
SHIN, T [1 ]
MURAO, S [1 ]
机构
[1] KUMAMOTO INST TECHNOL, FAC ENGN, DEPT APPL MICROBIAL TECHNOL, KUMAMOTO 860, JAPAN
关键词
D O I
10.1271/bbb.59.143
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The effects of alpha- and beta-arbutin on the activity of tyrosinases from mushroom and mouse melanoma were examined. alpha a-Arbutin was synthesized from hydroquinone and starch using glucoside synthetase (GSase). beta-Arbutin inhibited both tyrosinase activities from mushroom and mouse melanoma. alpha-Arbutin inhibited only the tyrosinase from mouse melanoma, 10 times as strongly as beta-arbutin. The IC50 of alpha-arbutin was 0.48 mM and its inhibitory mechanism was speculated to be mixed type inhibition, while that of beta-arbutin was noncompetitive.
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页码:143 / 144
页数:2
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