ENZYMIC OMEGA-OXIDATION - STOICHIOMETRY OF OMEGA-OXIDATION OF FATTY ACIDS

被引：17

作者：

PETERSON, JA

MCKENNA, EJ

ESTABROOK, RW

COON, MJ

机构：

[1] Department of Biophysics and Physical Biochemistry, Johnson Research Foundation, University of Pennsylvania, Philadelphia

[2] Department of Biological Chemistry, The University of Michigan, Ann Arbor

来源：

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS | 1969年 / 131卷 / 01期

基金：

美国国家科学基金会; 美国国家卫生研究院;

关键词：

D O I：

10.1016/0003-9861(69)90128-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The stoichiometry of the ω-oxidation of fatty acids catalyzed by the inducible enzyme system of Pseudomonas oleovorans was determined. Assay methods are described for the polarographic measurement of oxygen uptake and the spectrophotometric measurement of DPNH oxidation. The development of these assay methods for the ω-hydroxylase has permitted the determination of the stoichiometry of the ω-hydroxylation of fatty acids. The reaction is a mixed function oxidation and the following equation is sufficient to describe the stoichiometry of the reaction catalyzed by the enzyme system: DPNH + H+ + laurate + O2 → DPN+ + H2O + ω-hydroxylaurate. © 1969.

引用

页码：245 / +

页数：1

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