CONTROL OF SUGAR UTILIZATION IN ORAL STREPTOCOCCI - PROPERTIES OF PHENOTYPICALLY DISTINCT 2-DEOXYGLUCOSE-RESISTANT MUTANTS OF STREPTOCOCCUS-SALIVARIUS

被引：31

作者：

GAUTHIER, L ^{[1
]}

BOURASSA, S ^{[1
]}

BROCHU, D ^{[1
]}

VADEBONCOEUR, C ^{[1
]}

机构：

[1] UNIV LAVAL,ECOLE MED DENT,ST FOY G1K 7P4,QUEBEC,CANADA

来源：

ORAL MICROBIOLOGY AND IMMUNOLOGY | 1990年 / 5卷 / 06期

关键词：

phosphotransferase system; phosphotransferase system mutant; streptococci; sugar utilization;

D O I：

10.1111/j.1399-302X.1990.tb00440.x

中图分类号：

R78 [口腔科学];

学科分类号：

1003 ;

摘要：

The physiological and biochemical characterization of Streptococcus salivarius mutants isolated by positive selection for resistance to 0.5 mM 2‐deoxyglucose in the presence of lactose are reported. We found 2 classes of mutants following a series of experiments that included: growth rate determinations, uptake studies, measurement of phosphotransferase system (PTS) activities and detection of the IIIman proteins by Western blotting and analysis of [32P]PEP‐phosphorylated proteins. Class 1 mutants did not possess the low‐molecular‐weight form of IIIman. They did not grow on mannose and were unable to transport 2‐deoxyglucose. On the other hand, class 2 mutants possessed the 2 forms of IIIman, grew readily on mannose and transported 2‐deoxyglucose, albeit at a lower rate than the parental strain. Both classes of mutants exhibited abnormal growth in media containing mixtures of sugars. Moreover, derepression of genes coding for catabolic enzymes was observed in all the mutant strains. Our data suggested that the role of the mannose PTS in the control of sugar utilization in S. salivarius is complex and may involve the participation of several components. Copyright © 1990, Wiley Blackwell. All rights reserved

引用

页码：352 / 359

页数：8

共 39 条

[1] Chin MA, Feldhein DA, Saier MH, Altered transcriptional patterns affecting several metabolic pathways in strains of Salmonella typhimurium which overexpress the fructose regulon, J Bacteriol, 171, pp. 2424-2434, (1989)
[2] Deutscher J, Sossna G, Gonzy-Treboul G, Regulatory functions of the phospho‐carrier protein HPr of the phosphoenol‐pyruvate‐dependent phosphotransferase system in gram‐positive bacteria, FEMS Microbiol Rev, 63, pp. 167-174, (1989)
[3] Dills SS, Apperson A, Schmidt MR, Saier MH, Carbohydrate transport in bacteria, Microbiol Rev, 44, pp. 385-418, (1980)
[4] Dills SS, Seno S, Regulation of hexitol catabolism in Streptococcus mutans, J Bacteriol, 153, pp. 861-866, (1983)
[5] Gachelin G, Studies on the α‐melhylglucoside permease of Escherichia coli, Eur J Biochem, 16, pp. 342-357, (1970)
[6] Garvey JS, Cremer NE, Sussdort DH, Methods in immunology, pp. 313-321, (1977)
[7] Hamilton IR, Effect of changing environment on sugar transport and metabolism by oral bacteria, Sugar transport and metabolism by gram‐positive bacteria, pp. 94-133, (1987)
[8] Hamilton IR, Lo CGY, Co‐induction of β‐galaclosidase and the lactose‐P‐enolpyruvate phosphotransferase system in Streptococcus salivarius and Streptococcus mutans, J Bacteriol, 136, pp. 900-908, (1978)
[9] Laemmli UK, Cleavage of structural proteins during the assembly of the head of bacteriophage T4 Nature, 227, pp. 680-685, (1970)
[10] Liberman ES, Bleiweis AS, Role of the phosphoenolpyruvate‐dependent glucose phosphotransferase system of Streptococcus mutans GS‐5 in the regulation of lactose uptake, Infect Immun, 43, pp. 536-542, (1984)

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