CLONING AND CHARACTERIZATION OF THE GENE ENCODING TRYPANOSOMA-CRUZI DNA TOPOISOMERASE-II

被引：54

作者：

FRAGOSO, SP ^{[1
]}

GOLDENBERG, S ^{[1
]}

机构：

[1] FDN OSWALDO CRUZ, DEPT BIOQUIM & BIOL MOLEC, EXPRESSAO GEN LAB, AVENIDA BRASIL 4365, BR-21045 RIO DE JANEIRO, RJ, BRAZIL

来源：

MOLECULAR AND BIOCHEMICAL PARASITOLOGY | 1992年 / 55卷 / 1-2期

关键词：

DNA TOPOISOMERASE-II; TRYPANOSOMA-CRUZI; CHAGAS DISEASE; GENE CLONING;

D O I：

10.1016/0166-6851(92)90133-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The gene encoding Trypanosoma cruzi type II topoisomerase (TcTOP2) was isolated from a genomic library with a heterologous probe corresponding to part of the Trypanosoma brucei type II topoisomerase (TBrTOP2) gene. Nucleotide sequencing of TcTOP2 showed that the gene consists of an open reading frame of 3696 nucleotides (1232 amino acids), predicting a polypeptide product of 138 413 Da. Comparison of the amino acid sequence with that of type II topoisomerases from T. brucei (TBrTOP2) and Crithidiafasciculata (CfaTOP2), shows a high degree of conservation with estimated identities of 78% and 69%, respectively. TcTOP2 is a single copy gene in the genome of T. cruzi Dm28c and is expressed as a 4.5-kb mRNA. PCR mapping showed two distinct mini-exon addition sites at positions 225 and 203 upstream from the initiator AUG.

引用

页码：127 / 134

页数：8

共 37 条

[1] CHROMOSOME ASSEMBLY INVITRO - TOPOISOMERASE-II IS REQUIRED FOR CONDENSATION [J].