EFFECTS OF TRIFLUOROACETIC-ACID, A HALOTHANE METABOLITE, ON C6 GLIOMA-CELLS

Effects of trifluoroacetic acid (TEA) on cell growth, DNA, glycoprotein, and dolichol- linked oligosaccharides synthesis and ribonucleotide triphosphate concentrations were examined in exponentially growing C6 murine gtioma cells. One day of treatment with TFA caused a slight concentration-dependent enhancement of cell growth and [3H]thymidine incorporation. Exposure for 1 or 5 d to TFA (0.5-7.0 mM) elevated the [3H]leucine incorporation in a dose- and time-dependent manner. The results suggested that TFA stimulated cell growth and enhanced protein synthesis. TFA also affected [3H]mannose incorporation into glycoproteins and dolichol-linked oligosaccharides in a dose-dependent fashion. In addition, it was found that TFA accelerated lectine-induced cell agglutination. These data suggest that TFA, the principle halo- thane metabolite, alters plasmalemmal glycoprotein synthesis. These findings should form a basis for further understanding on the mechanism underlying halothane- associated neurotoxicity. © 1975 Taylor & Francis Group, LLC.